CLOTHING METHOD
receiving eggs from the couple

ROPA method receiving eggs from the couple
WHAT IS THE ROPA METHOD?
The ROPA method, also known as double motherhood, means Reception of Ovules from the Couple and is a variant of In Vitro Fertilization intended for couples formed by two women. In this context, one of the women of the couple undergoes ovarian stimulation to provide the eggs, and the embryos obtained are transferred to the other woman of the couple. In this way, both are mothers, thus achieving a total involvement of both in the motherhood project.
The ROPA method,
a unique and shared experience.
The ROPA method is exclusive to couples formed by two women who want to actively participate in the experience of motherhood from the beginning. It is one of the existing techniques that homosexual couples can use to become mothers.
The ROPA Method Process Reception of Eggs from the Couple



FIRST CONSULTATION
The first step to starting this project together is to arrange an initial visit with one of our gynecologists specializing in fertility, to carry out a comprehensive study of your medical and reproductive history, determine the necessary tests to decide what role each of the women in the couple can take in this type of In Vitro Fertilization and together chart the best path to motherhood.
OVARIAN STIMULATION


PREPARATION OF THE UTERUS
The endometrium is the layer of the uterus that must receive and host the embryo. It is equally important that its preparation is correct. For this to be possible, it is necessary to have previously synchronized the two members of the couple so that they are in the same phase of the menstrual cycle. The objective of this synchronization is that, when the mother who provides the eggs is ready for extraction, at the same time, the mother who will receive the embryos, has her uterus prepared to host the embryo. To do this, the recipient mother who is to receive the embryo must also follow the minimum medication regimen and undergo ultrasound checks.

EGGS EXTRACTION
The extraction of the eggs or follicular puncture is a process that is carried out in the operating room of the center under sedation. It is a simple procedure, which lasts between 15 and 20 minutes. With a needle attached to a transvaginal ultrasound probe, the aspiration of all the follicles is performed, collecting their contents in laboratory tubes.
After 1-2 hours of the puncture, the patient is discharged and home rest is recommended for the rest of the day.
IN VITRO FERTILIZATION LABORATORY
Identification and collection of eggs
At the same time that the follicular puncture is being performed, Embriogyn biologists are already searching the liquids obtained for the eggs that will be used to carry out the in vitro fertilization process.
These eggs are kept in incubators to maintain them in the physiological conditions that guarantee their good development. At this first point, traceability control of the gametes and future embryos begins using the Witness system, marking the plates containing the eggs with the couple’s identification card.
Semen preparation
In the case of the ROBA method, the semen used for in vitro fertilization is a donor’s semen. The sperm samples used are from anonymous donors from a duly accredited Semen Bank. The samples are kept frozen and meet the requirements established by the current law regarding cell and tissue banks. The law regulates that the Bank must ensure that it offers semen with the greatest possible similarity in terms of physical characteristics, blood group and Rh. Sperm donation is a free, anonymous and free act of a man of legal age whose psychophysical state meets the requirements of a mandatory donor study protocol.
In the Andrology Laboratory, the sperm sample will be thawed and processed with the most appropriate sperm training technique to select the best sperm to perform fertilization techniques.
Fertilization techniques
There are two techniques for carrying out fertilization:
- Conventional in vitro fertilization (conventional IVF): is the technique in which previously treated eggs and sperm are placed together, so that fertilization occurs spontaneously and naturally.
- ICSI (Intra Cytoplasmic Sperm Injection): consists of introducing a sperm directly into the mature egg with the help of a microscope and a micromanipulation system.
Fertilization assessment
To verify that fertilization has taken place correctly, it is necessary to wait a few hours to see the first signs of genetic activation of the egg and sperm. After about 16-20 hours, it is possible to verify whether fertilization has been correct by visualizing the two characteristic structures: two polar corpuscles and two pronuclei. The other possible structures that can be observed must be discarded, as they do not correspond to correct fertilization. At this point, we can already know the number of embryos generated and the biology team will contact the couple to update the information obtained.
Embryo culture, the EMBRYOSCOPE
In the days following fertilization, the embryos will be kept in culture in incubators for between 3 and 6 days. At Embriogyn we have the EmbryoScope, which is a state-of-the-art incubator that allows images of embryonic development to be obtained at all times. Its use provides great information on the embryonic division process, thus helping to select the best embryo to transfer to the maternal uterus and increasing the chances of pregnancy by up to 15-20%. Every day, information on the evolution of the embryos will be communicated and transferred to the couple so that they are informed at all times of the state of their embryos.
EMBRYO TRANSFER
Once the best embryo has been selected, it will need to be deposited in the uterus of the recipient woman. Embryo transfer is a simple and painless procedure in which the embryo is placed in the fundus of the uterus with the help of a catheter specifically designed for this purpose. We recommend that after this process, rest in the Center for about 15 minutes and relative rest at home until the day after the transfer.
Embryo transfer can be of 1 or 2 embryos, depending on the characteristics of each couple. It is a consensus decision between the couple and the medical team, taking into account the history of each particular case, in order to offer the greatest probability of pregnancy and minimize the risk of multiple pregnancy.


VITRIFICATION OF THE REMAINING EMBRYOS
In the event that there are more good quality embryos that have not been transferred, they will be kept frozen for future use thanks to the vitrification technique.
Vitrification offers very high survival rates in the freezing/thawing process, which allows the possibility of having more than one embryo transfer with a single in vitro fertilization process without the need to restart ovarian stimulation.
PREGNANCY TEST AND ULTRASOUND
In the days following embryo transfer (beta-waiting), we recommend leading a completely normal life, without major physical exertion, and maintaining a positive attitude. Going out, staying busy, and having fun will help you enjoy this period more.
The pregnancy test is performed 14 days after the date of egg retrieval. It is a test that can be done at home, in an intimate and comfortable way, through a pregnancy test with the first urine of the morning.
If the result is positive, we will make an appointment three weeks later to have an ultrasound to check that the pregnancy is progressing correctly.

WHAT DOES THE TREATMENT INCLUDE?
- Hormone analyses during the cycle
- Necessary medical visits and hormonal stimulation checks
- Follicular puncture and anesthesia
- Processes in the IVF laboratory
- Semen bank selected by the specialized team
- Processes in the andrology laboratory
- ICSI (Sperm Microinjection) / IVF
- Embryo culture with Embryoscope technology
- Embryo transfer
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B-HCG pregnancy test
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Pregnancy check ultrasound
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Witness system for security and traceability of biological samples
TREATMENT PRICE: €5,415
MAKE AN APPOINTMENT
It’s quite simple. All you have to do is contact Embriogyn and make an appointment with our specialists at the time that suits you best. In case you can’t come in person to the clinic, visits can also be arranged via Skype.